The Lakes of Missouri Volunteer Program -
Related Research

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Missouri

Missouri Stream Teams tested Coliscan Easygel and the membrane filter technique with diluted waste water influent. They used dilutions to do monthly QA/QC on their membrane filter technique for fecal coliform bacteria. Volunteer Monitoring Coordinator, Tim Reilly told us he "did splits from the dilutions with both methods, and replicates with each method and compare[d] the results". He found that the results did not compare. He said, "the membrane filter technique derived higher numbers of fecal coliform /100mL" and that the Coliscan plate was hard to read. For more information about this program-level study, contact Tim Reilly at: Tim.Rielly@mdc.mo.gov

Tony Thorpe of the Lakes of Missouri Volunteer Program has assessed Coliscan Easygel. They compared Easygel results vs. traditional plated and incubated methods. Tony reported on the EPA listserver that "qualitative results show that Easygel underestimates E.coli by around 50%, but that was with an "n" of 3, all from the same stream (though different locations)". Based on these results, volunteers in the Lakes of Missouri program who are using Easygel at this point in time are doing so with the understanding that until complete accuracy testing is done, the results indicate presence/absence only. For more information, contact Tony Thorpe at: thorpet@missouri.edu

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Missouri Department
of Natural Resources

Missouri Watershed Information Network (MoWIN)
Send comments to: mowin1@missouri.edu
205 Agricultural Engineering
Columbia, MO 65211
Phone: (573) 882-0085
Toll Free: (MO only): 1-877-H20-shed (426-7433)
Fax: (573) 884-5650

Page last updated August 26, 2008